DMT Presenter Control Bedienungsanleitung Seite 52

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52
Culture Myograph System Model 202CM
• The branch points of arteries are V-shaped whereas those of veins are more
U-shaped.
• The arterial wall contains a thick layer of smooth muscle cells compared to
the vein wall, which only contains a single or a few layers of smooth muscle
cells. The histological difference is clearly visible in the stereo microscope.
• If you still have difculty and the vein and artery still contain some blood then
try to move the blood forward by very gently squeezing the vessels with a forceps.
In the artery the blood will run back quickly whereas in the vein the blood will run
back very slowly if it even does so. Note, it is important that you perform this on
vessels other than those you will use as this procedure damages the vessels.
8. Dissect away the vein using scissors to cut the adipose and connective tissue
between the artery and vein. One method is to cut the vein in one position
and afterwards gently to pull the vein away from the artery. In this way a ne
membrane of connective tissue becomes visible between the adipose tissue
and the artery. Carefully cut the ne membrane to remove the vein and adipose
tissue while avoiding any direct contact between the scissor and artery (Fig. 7.4A-
B).
Figure 7.3 Distinguishing between artery and vein
Vein
Artery
“V-shaped”
“U-shaped”
Figure 7.4 Removal of vein
A B
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