
36
Culture Myograph System Model 202CM
5. Once the dimensions and units of the calibrated rectangle are correctly
entered, click the ”calibrate” button on the dialog box. The VediView
™
image
analysis is now calibrated. All parameters passed to other control bars or
logged will be calibrated.
6. To save the calibration settings for future experiments, a calibration le
(*.cal) can be saved and subsequently loaded from the Calibration dialog
box. The le name being used for calibration is displayed in the dialog box; to
remind the user of the current settings. The user may also change the scaling
applied to the current calibration parameters from the calibration dialog box
or by using the "adjust calibration" icon on the tool bar. This facility can
be useful if VediView
™
is used with a video-microscope system in which the
magnication settings are changed. If for example VediView
™
is calibration
using an x20 microscope objective and subsequently an x50 device replaces
the objective, then the calibration can be adjusted by a suitable factor (in this
case 2.5). Given the optical factors affecting such a change, it is always worth
checking the calibration for obtaining the most accurate results.
3.5 Customising the Interface
The functionality of VediView
is based on a series of control bars that is congurable
in different ways. The interface can be changed to suit a particular experiment or
user. Once the Interface has been designed it may be saved for any future use. The
default interface setup (setup 0) is initially set to the factory default values. Although
set-ups can be redened, the factory defaults can never be changed. A custom
interface can be designed and implemented by following the procedure:
1. Launch VediView
™
and select the user setup from the View menu. If this is the
rst time you are using VediView
™
then the interface set-up will be equal to
the factory setting. Note that you can always return to the factory settings by
selecting factory setting from the options in the user setup.
2. Design of a custom
Figure 3.27 VediView™ Main
window with factory settings
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