DMT Presenter Control Bedienungsanleitung PDF herunterladen (Seite 32)

Culture Myograph System Model 202CM

Data logging is not automatic and must be activated by selecting (or creating) a le.

Files are chosen by clicking the “start data logging” button in the data control bar

or by selecting the “start logging data” menu item in the pop-up menu (click on )

on the data control bar. When data logging is active the “cross” indicator becomes

a “tick” indicator . Data logging can be stopped at any time by selecting the “stop

data logging” button in the data control bar or by selecting the “start/stop logging

data” menu item in the pop-up menu. Note that data will be stored in the data history

graph regardless of whether data is logged to disk.

3.3.4 Analysis

The analysis control bar is used to activate and determine the type of analysis to

be performed on the acquired images. The purpose of this analysis is to determine

the dimensions of the vessel in the myograph and to derive other parameters (e.g.

vessel cross section) based upon those dimensions. Although the video microscope

supplies the image for this analysis, some judgements must be made concerning

the analysis region and the nature of the analysis. VediView

™

uses a unique vessel

detection and analysis (VDA) methodology to recognise the presence of a vessel and

locate its edges. This method is largely automatic and effectively checks the correct

imaging of the vessel and is capable of estimating the position of lumen edges even

when the vessel is contracting (when conventional edge detection can not be used).

Since the analysis is implemented in real time on live images, the user can interact

with and recongure the analysis so that an optimum setting can be achieved for the

given vessel and microscope being used. VediView

™

provides this feature to the user

without them requiring any specialist knowledge of the image processing algorithms

being implemented. The pop-up menu on the control bar (select ) can be used to

access all functions of the analysis bar. The pop-up menu can also be revealed by a

right mouse button click when the Windows cursor is above the control bar. A more

detailed description of the functions available in the analysis control bar follows

under these sub-headings:

• Setting the Analysis Rectangle

• Calibration of Image Analysis

• Starting Real Time Analysis

• Conguration of Vessel Detection and Analysis (VDA)

• Logging and Reviewing Results of Analysis

3.3.4.1 Setting the Analysis Rectangle

If the user requires a calibrated image analysis, the dimensions of the image must

be determined before commencing analysis. To perform this calibration the user

is referred to How To... Calibrate the Image Analysis. It is now assumed that the

calibration (if required) has already been performed. Before selecting a region for

analysis, the image should be displayed in the main window. Drag and click the

mouse to select a rectangle in the main window and then select the “Set analysis

rectangle” icon on the tool bar. The analysis rectangle may be re-selected by

simply drawing a second rectangle and re-selecting the “Set analysis rectangle” icon

. These processes may be repeated until the user is satised with the selected

region.

Figure 3.16

Analysis control bar

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