DMT Presenter Control Bedienungsanleitung PDF herunterladen (Seite 33)

3.3.4.2 Calibration of Image Analysis

Calibration of image dimensional analysis can be done in sensible units (e.g. microns)

dened by the user. To perform such a calibration the user is referred to How To...

Calibrate the Image Analysis. The default calibration is performed in units dened by

the image data resolution (i.e. pixels).

VediView

™

can calculate both ow rate and vascular resistance but it requires

knowledge about the uid viscosity and vessel length. These values must be entered

in the "analysis conguration" dialog box accessed from the tool bar icon or from

the pop up menu. The default viscosity value

is the one for water, which is suitable for the

majority of myograph experiments. The vessel

length can be measured by using VediView

™

or any stereo microscope equipped with an

eyepiece reticule.

3.3.4.3 Starting Real Time Analysis

Before starting the image analysis it is necessary to select the analysis rectangle (see

above). If the user wishes to obtain calibrated measures of the vessel dimensions, a

procedure must be followed as explained in How To... Calibrate the Image Analysis. To

commence image analysis select the “stop/start analysis” icon on the tool bar or

select the “Start image analysis” menu item from the pop up menu (click on ) on

the analysis control bar.

3.3.4.4 Conguration of Vessel Detection and Analysis (VAD)

The dimensional analysis of vessel images is automatic and contains an algorithm

that effectively checks the correct positioning of the vessel and its analysis window.

If the vessel image within the analysis rectangle clearly shows the vessel walls with

good contrast, the algorithm will have no trouble in nding the edges and monitoring

their changes throughout an experimental protocol. This condition will be clear from

the analysis results window showing a clearly delineated “edge prole” (Fig. 3.18 and

3.19).

If the analysis rectangle does not contain a “valid” vessel, VediView

™

will indicate that

no edge detection is possible (Fig. 3.20 and 3.21). This condition will be clear from

the analysis results window showing the "cross" indicator .

Figure 3.17 Conguration of vessel

detection and analysis

Figure 3.18 Analy-

sis rectangle

Figure 3.19

Vessel wall analysis

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