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5.3 Pressure Regulator

The Pressure Regulator is connected to

the culture myograph system to generate a

pressure on the mounted vessel from the

perfusion buffer. The pressure is adjusted using

the knob and air release valve shown in Fig. 5.6.

5.4 Culture Myograph Maintenance

The Culture Myograph System – Model 202CM is a very delicate and sophisticated

piece of research equipment. In order to keep it working at its best, DMT recommend

that the following sections are read carefully and that the instructions are followed at

all times.

DMT strongly recommends that the myograph chamber and surroundings be cleaned

after each experiment.

After a “normal” experiment use the following procedure to clean the myograph

chamber and glass cannulas:

1. Fill up the myograph chamber to the edge with an 8% acetic acid solution and

allow it to stand for a few minutes to dissolve calcium deposits and other salt

build-up. Use a swab stick to mechanically clean all chamber surfaces.

2. Remove the acetic acid and wash the myograph chamber and glass cannulas

several times with double distilled water.

3. It is hard to remove any kind of hydrophobic reagent used by using step

1. and 2., try incubating the chamber and glass cannulas with 96% ethanol or

a weak detergent solution (e.g. Treepol).

4. To remove more resistant or toxic chemicals, incubate the myograph chamber

and glass cannulas with 1M HCl for up to 1 hour. In exceptional cases incubate

the chamber and supports with a up to 3M HNO

solution for about

15 minutes.

5. Wash the myograph chamber and glass cannulas several times with double

distilled water.

Important Notes:

• To sterilize the culture myograph chamber and glass cannulas, use a standard

autoclave procedure.

• Be very careful using step c) and d) repeatedly as strong reagents can cause

extreme damage to the myograph unit.

• Be very careful not to damage the glass cannulas during the cleaning procedure.

Figure 5.6 Pressure Regulator

Culture Myograph System Model 202CM

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DMT Presenter Control Bedienungsanleitung Seite 46